abstract
BACKGROUND:
Some
polyphenols induce apoptosis and inhibit angiogenesis. Consumption of
black tea, rich in polyphenols, has been found to reduce ovarian cancer
risk. Theaflavin (TF1), theaflavin-3-gallate (TF2a),
theaflavin-3'-gallate (TF2b) and theaflavin-3, 3'-digallate (TF3) are
four main theaflavin derivatives found in black tea.
MATERIALS AND METHODS:
Cell
proliferation assay, Hoechst 33342 staining assay, Caspase-Glo Assay,
western blot, human umbilical vein endothelial cell tube formation assay
and vascular endothelial growth factor (VEGF) enzyme-linked
immunosorbent assay were performed.
RESULTS:
All
four theaflavin derivatives reduced viability of ovarian cancer cells
at lower concentrations than with normal ovarian cells. TF1 mainly
mediated apoptosis via the intrinsic pathway, while the others via the
intrinsic and extrinsic pathways. TF1 inhibited tube formation via
reducing VEGF secretion in a hypoxia-inducible factor 1α-independent
manner, while the others in a HIF1α-dependent way.
CONCLUSION:
All
four
theaflavin derivatives inhibited ovarian cancer cells. Some of the
effects and mechanisms of TF1 are different from those of the other
three theaflavin derivatives.
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