BACKGROUND:
Polyneuropathy
is defined as a simultaneous malfunction of several peripheral nerves,
which could be a toxic side effect of cancer therapy; however, this is
reported to occur rarely and difficult to confirm.
AIMS:
The
purpose of the study was to introduce, at the first time, the
calorimetry in the diagnosis of
neuropathy in an
experimental animal
model.
The study was inspired by the forensic investigation of a
53-year-old cancer survivor female patient, in whom the development of
polyneuropathy could be caused by cyclophosphamide therapy.
METHODS:
Adult
guinea pigs were injected intraperitoneally with the dose of
cyclophosphamide, comparable to the human dosage. Animals were
euthanized, then nerve and muscle samples were analyzed using a SETARAM
Micro calorimeter. Denaturation temperatures were measured and the
calorimetric enthalpies were calculated based on the areas under thermal
absorption curves.
RESULTS:
Thermal denaturations of the
samples were decreased and the calorimetric enthalpies were increased
depending on the doses of cyclophosphamide. The nerves were more
sensitive to chemotherapy as compared to the muscles.
CONCLUSION:
It
is concluded that toxic effects of cyclophosphamide on peripheral
nerves and muscles can be measured and analysed by calorimetry, and that
the effect was
dose dependent.
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