abstract
Source
State Key Laboratory of Food Science and Technology, Nanchang University, Jiangxi, 330047, China; Penn Ovarian Cancer
Research Center, Center for Research on Reproduction and Women's Health
(CRRWH), Department of Obstetrics and Gynecology, University of
Pennsylvania, Philadelphia, PA, 19104, USA; Institute of Translational
Medicine, Nanchang University, People's Republic of China, 999 Xuefu Rd,
Honggu District, Nanchang, China.
BACKGROUND:
Probiotics
are widely used in the food industry and medicine fields in China, but
few studies have been conducted to evaluate the actual microbial amounts
and species in probiotic products, which may conflict with the labels
and mislead consumers to choose inappropriate foods or medicines.
RESULTS:
Twenty
commercial dairy products and eight commercial 'healthcare' samples
were collected from markets in China and tested using culture-dependent
and culture-independent methods. The results suggested that the total
bacterial counts of most commercial products met the minimum
quantitative requirement of the Chinese national standard (6.00 log
colony-forming units g(-1) ). However, the bacterial counts of specific
species were inconsistent with the labelling. In parallel, denaturing
gradient gel electrophoresis analysis indicated that some
probiotic-containing products were wrongly labelled; no Bifidobacterium
species were detected in the products claiming to contain
bifidobacteria, and the probiotic characteristics (antimicrobial
activity, acid resistance and bile resistance) of some isolates had
degraded. Moreover, some contaminating bacteria, e.g. Enterobacter sp.,
Klebsiella sp. and Serratia sp., were also detected in these products.
CONCLUSION:
The
combination of culture-dependent and culture-independent methods was
proven to quickly and conveniently detect the microbial diversity in
probiotic products, and more effort is required to regulate the
probiotic market in China
0 comments :
Post a Comment
Your comments?
Note: Only a member of this blog may post a comment.