Low uptake of fluorodeoxyglucose in PET/CT in ovarian clear cell carcinoma may reflect glutaminolysis of its cancer stem cell-like properties Ovarian Cancer and Us OVARIAN CANCER and US Ovarian Cancer and Us

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Tuesday, January 24, 2017

Low uptake of fluorodeoxyglucose in PET/CT in ovarian clear cell carcinoma may reflect glutaminolysis of its cancer stem cell-like properties



 What is glycolysis Where does it occur and what does it produce?
"Glycolysis" means the splitting of glucose (sugar). The first step of the process occurs in the cytoplasm of the cell and yields a small amount of energy (measured in units of ATP) and two molecules of pyruvate. The other steps occur in the mitochondria. It is an ongoing process as your cells have a constant need for energy.
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abstract (Japan)
Low uptake of fluorodeoxyglucose in positron emission tomography/computed tomography in ovarian clear cell carcinoma may reflect glutaminolysis of its cancer stem cell-like properties

  In conclusion, we suggested that ovarian CCC showed low activation of glycolysis, and this may reflect glutaminolysis of its CSC-like properties.
The characteristics of ovarian cancers that showed low activation of glycolysis were investigated. Using medical records of patients with ovarian cancers who had undergone fluorodeoxyglucose positron emission tomography/computed tomography (FDG-PET/CT) prior to their primary surgery at the University of Tokyo Hospital between 2010 and 2015, we identified cases with a low uptake of FDG in PET/CT.
We considered the maximum standardized uptake value (SUVmax) as the degree of glucose uptake. We investigated the properties which may account for the low activation of glycolysis in vitro. The expression level of alanine, serine, cysteine-preferring transporter 2 (ASCT2, a glutamine influx transporter), system L-type amino acid transporter 1 (LAT1, a glutamine efflux transporter) and glucose transporter 1 (GLUT1, a glucose influx transporter) were investigated by western blotting. The phosphorylation level of AMP-activated protein kinase (AMPK), which is one of the metabolic sensors, was also investigated. Most of the cases with a low uptake SUVmax were limited to patients with ovarian clear cell carcinoma (CCC). We obtained cancer stem cell (CSC)-like properties from CCC cell lines, and compared the expression levels of transporters between non-CSCs and CSCs. Whereas the expression level of ASCT2 was nearly unchanged between non-CSCs and CSCs, the expression levels of LAT1 and GLUT1 were decreased in CSCs compared to non-CSCs. The phosphorylation level of AMPK was reduced in CSCs compared to non-CSCs. In conclusion, we suggested that ovarian CCC showed low activation of glycolysis, and this may reflect glutaminolysis of its CSC-like properties.

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