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abstract
Background and Aims
Microsatellite
instability (MSI) in colorectal cancer (CRC) cells results from
deficient mismatch repair (MMR) protein function, either acquired or
from germline alterations, such as in patients with Lynch Syndrome.
Universal screening initiatives for Lynch Syndrome have been encouraged.
However, little is known about the true prevalence of MMR deficiency
and MSI in colorectal tumors among individuals from different racial and
ethnic subgroups or their clinical effects in these populations.
Methods
We
performed a retrospective analysis of 253 surgically resected, primary
colorectal adenocarcinoma specimens identified from the University of
Miami tumor registry from 2005 through 2010. We collected clinical data,
including overall survival (OS), the proportion of patients alive at
specific intervals, from non-Hispanic White, Hispanic, and Black
patients matched by stage. We performed immunohistochemical staining to
detect MMR proteins in all specimens and PCR analysis of 51 tumors to
detect MSI.
Results
We
detected MMR deficiency in 28/253 cases (11.1%), evenly distributed
among Blacks (9.6%), non-Hispanic Whites (10.4%), and Hispanics (12.6%) (P=.79).
Combined deficiencies in MLH1 and PMS2 were found in 23/28 of MMR
deficient samples (82.1%); MSH2 and MSH6 were most frequently absent in
tumor samples from Hispanics (P=.03). Eleven of 51 tumor
samples (21.6%) had high levels of MSI, and we observed a high level of
concordance between MMR and MSI (k=.81). OS was significantly better in
patients whose tumors had deficient MMR (hazard ratio for patients with
MMR deficient tumors vs MMR proteins intact=0.37; 95% confidence
interval, 0.15–0.91; P=.03). Race and ethnicity were not significant predictors of OS.
Conclusions
MMR
deficiency in colorectal tumors occurs with similar rates among
patients of different racial and ethnic groups, based on an
immunohistochemical analysis of 253 primary tumor specimens. This
finding indicates the potential value of universal testing of CRC by
immunohistochemistry in minority populations and confirms the benefit of
MMR deficiency to OS.
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