New imaging method reveals nanoscale details about DNA: Enhancement to super-resolution microscopy shows orientation of individual molecules

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New imaging method reveals nanoscale details about DNA: Enhancement to super-resolution microscopy shows orientation of individual molecules
June 17, 2016

Researchers have developed a new enhanced DNA imaging technique that can probe the structure of individual DNA strands at the nanoscale. Since DNA is at the root of many disease processes, the technique could help scientists gain important insights into what goes wrong when DNA becomes damaged or when other cellular processes affect gene expression.

The new imaging method builds on a technique called single-molecule microscopy by adding information about the orientation and movement of fluorescent dyes attached to the DNA strand.....
 
Journal Reference:

1. Adam S. Backer, Maurice Y. Lee, W. E. Moerner. Enhanced DNA imaging using super-resolution microscopy and simultaneous single-molecule orientation measurements. Optica, 2016; 3 (6): 659 DOI: 10.1364/OPTICA.3.000659