abstract
Highlights
- •Cytotoxic CD8+ T cells and CD20+ B cells play a critical role in ovarian cancer.
- •Markers for tumor infiltrating lymphocyte homing and function have survival benefits.
- •Presence of HLA Class II staining translates directly to ovarian cancer prognosis.
Abstract
Objective
Epithelial
ovarian cancer (EOC) is the most lethal gynecologic malignancy. Several
factors prognostic for survival have been identified including the
presence of certain lymphocyte markers. Tumor-infiltrating lymphocytes
(TILs), particularly cytotoxic CD8+ TILs, have been shown to be most favorable for prognosis in ovarian cancer, although other immune cells including CD3+ T-cells, CD4+
T-cells, and B-cells have also demonstrated survival benefits. Although
data for these markers exists, results are not uniform in the
literature. Furthermore, other immunomodulatory protein markers that
have been targeted in effective immunotherapies for other malignancies
may prove to be favorable in ovarian cancer.
Methods
Here,
extensive immunohistochemical analysis was performed on a tissue
microarray, containing 135 ovarian cancer cases obtained during tumor
debulking detecting 15 key lymphocyte markers such as CD3, CD4, and
CD20, as well as activation and immunomodulatory molecules such as TIA-1
and PD-L1. Samples were analyzed for expression of markers in tumor
islets or stroma and expression was correlated with overall survival,
histotype, stage, age, debulking grade, and response to chemotherapy.
Results
Our results confirm the presence of CD8+ and CD20+
TILs is positively correlated with overall survival, with further
multivariate modeling replicating that prognostic benefit. Additional
markers of significant prognostic importance, including TIA-1, CD103 and
HLA Class-II were also revealed.
Conclusions
Our
results further support the vital role of cytotoxic T-cells in defense
against ovarian cancer and reveals new questions as to the role of
B-cells in tumor control as well as the potential benefits of
immunotherapy involving other immune modulating molecules.
0 comments :
Post a Comment
Your comments?
Note: Only a member of this blog may post a comment.